Project

Part:BBa_K2070005:Design

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-20)


Constitutive ecf35 generator with ecf35 inducible GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790
    Illegal BsaI.rc site found at 1543


Design Notes

This part was designed so the the functionality of the sigma promoter 35 and its cognate sigma factor could be tested.

Source

Asssembly

References